|
KMID : 1140120080130040256
|
|
Cancer Prevention Research
2008 Volume.13 No. 4 p.256 ~ p.261
|
|
|
|
|
Chung Seok-Won
|
|
|
Abstract
|
|
|
|
The tumor suppressor p53 has been known as a sequence-specific master regulatory gene that participates in an extensive transcriptional regulation providing for genome integrity in response to genotoxic stresses. p53 tumor suppressor has also been known to be involved in apoptosis, cell cycle arrest and DNA repair. However, there are some limitations to study detailed mechanisms. Recently, C.elegans has been reported to be a useful animal model for toxicology test as well as for the investigation of cellular responses. Indeed C.elegans, among the various species, about 40% of them are similar to human genes. In this study, we redesigned a p53 homologue (CEP-1) in C.elegans. The CEP-1 gene was cloned into L4440 DoubleT-7scriptII (RNAi feeding vector) for C.elegans. Our data showed that the significant reduction of growth in CEP-1 down-regulated C.elegans has been observed with comparison to in normal L4440 DoubleT-7scriptII feeding C.elegans suggesting that CEP-1 might have participated in the growth of C.elegans. Elucidation of p53 detailed mechanism in C.elegans homologue to human p53 would be needed to be explored in further study.
|
|
|
KEYWORD
|
|
|
p53, CEP-1 RNAi, C.elegans, Growth
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|